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. 2020 Oct 15;21(20):7640. doi: 10.3390/ijms21207640

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Liver mRNA expression of inflammatory markers (a) TNF-α, (b) IL-6, (c) MCP-1, (d) TLR-4, (e) IL-1β. Percentage of macrophage number as identified by (f) F4/80 and (g) CD68 immunohistochemistry staining and (h) and (i) respective representative images were taken at 10× magnification. Positive staining indicated by arrows in h and i. Data are represented as mean ± S.E.M and were analysed using two-way ANOVA followed by post hoc Fischer’s LSD test, n = 5–9. * p < 0.05 vs. Chow-PBS; ** p < 0.01 vs. Chow-PBS; # p < 0.05 vs. HFD-PBS; ## p < 0.01 vs. HFD-PBS.

Liver mRNA expression of inflammatory markers (a) TNF-α, (b) IL-6, (c) MCP-1, (d) TLR-4, (e) IL-1β. Percentage of macrophage number as identified by (f) F4/80 and (g) CD68 immunohistochemistry staining and (h) and (i) respective representative images were taken at 10× magnification. Positive staining indicated by arrows in h and i. Data are represented as mean ± S.E.M and were analysed using two-way ANOVA followed by post hoc Fischer’s LSD test, n = 5–9. * p < 0.05 vs. Chow-PBS; ** p < 0.01 vs. Chow-PBS; # p < 0.05 vs. HFD-PBS; ## p < 0.01 vs. HFD-PBS.