Figure 3.

Morphological and cytological identification of MOF1a. (a) The plant type of H1 (WT) and mof1a. Bars = 20 cm. (b,c) Mature panicles of WT/mof1a. Bars = 1 cm. (d,e) I₂/IK staining and TTC staining of WT/mof1a pollen grains at mature stage. (f) Mature embryo sac of WT/mof1a. 1, central cell; 2, antipodal cells; 3, polar nuclei; 4, synergid; 5, egg cell. Green bars = 100 µm. (g) Seed setting of WT and mof1a. Five plants of WT and mof1a were studied, respectively. P value was calculated using two-tailed Student’s t-test. “**” indicates that P value was less than 0.01. (h,i) Pollen viability (TTC) and pollen fertility (I₂/IK) of WT and mof1a based on three independent biological samples. (j) The mature embryo sacs fertility of WT and mof1a. n, numbers of embryo sacs observed. The error bars indicate the ±SE.