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. 2020 Oct 16;21(20):7676. doi: 10.3390/ijms21207676

Figure 1.

Figure 1

Hyperglycemia induced Spp1 transcriptional activity in renal proximal tubular epithelial cells. (a–c) Primary culture of proximal tubular epithelial cells (PTEC) derived from enhanced green fluorescent protein (EGFP)-Spp1 knock-in reporter mice. PTEC were cultured in 5 mM or 30 mM glucose conditions for 7 days. (a) Microscopic analysis of fluorescence of 4ʹ,6-diamidino-2-phenylindole (DAPI) and MitoSOX staining and of EGFP-Spp1 expression. (b) Mean fluorescence intensity of MitoSox in PTEC (n = 3 per group). (c) Osteopontin (OPN) in the culture supernatants was assessed by enzyme-linked immunosorbent assay (ELISA; n = 6 per group). Data are shown as means, and error bars depict SEM. * p < 0.05, *** p < 0.001.