Figure 6.

Silencing NHERF1 expression results in decreased p-NF-kB levels. Primary fetal membrane cells (A) AECs, (B) AMCs, (C) CMCs, (D) and CTCs were transiently transduced with NHERF1 small interfering RNA (siRNA) and nontargeted siRNA as a control. Representative blots of NHERF1 knockdowns is shown (left column) for respective primary cell type. NF-kB levels following activation with LPS for 15 min is shown in the right column. Representative blots for four different types of primary cells from three independent experiments are shown. The blots with NHERF1 and p-NF-kB were stripped and reprobed with the β-actin antibody for loading control.