Figure 7.

Effect of NHERF1 in production of inflammatory cytokines from primary cells treated by LPS. Control nontargeted siRNA and NHERF1 siRNA knockdown cells were treated with LPS for 48 h, and a multiplex assay was performed to estimate (A) IL-6, (B) IL-8, and (C) IL-10 levels in different primary cell supernatants. Data is mean ± SEM from three experiments. Statistical significance was determined by unpaired student t-test considering scrambled RNA as a control. * p < 0.05, ** p < 0.01, and *** p < 0.001.