Table 1. Properties of the Studied Capture Probe and Target Protein Pairs and Comparison of the Theoretical and Fitted KD Values.
aptamer/antibody | target | molecular weight (kDa) | literature KD | fitted KD (μM) |
---|---|---|---|---|
anti-his tag aptamer | D2 | 60 | ∼4.6 μM63 | 29 ± 8 (R2 = 0.9551) |
anti-his tag aptamer | tyrosinase | 35 | ∼4.6 μM63 | 31 ± 7 (R2 = 0.9731) |
anti-his tag antibody | tyrosinase | 35 | ∼10 nM64 | 24 ± 5 (R2 = 0.9420) |
anti-AGR2 aptamer | AGR2 | 22 | ∼13 nM60 | 21 ± 1 (R2 = 0.9951) |
anti-protein A aptamer | protein A | 45 | ∼0.522 μM62 | 14 ± 1 (R2 = 0.9177) |