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. 2020 Sep 8;5(10):3058–3069. doi: 10.1021/acssensors.0c00670

Table 1. Properties of the Studied Capture Probe and Target Protein Pairs and Comparison of the Theoretical and Fitted KD Values.

aptamer/antibody target molecular weight (kDa) literature KD fitted KD (μM)
anti-his tag aptamer D2 60 ∼4.6 μM63 29 ± 8 (R2 = 0.9551)
anti-his tag aptamer tyrosinase 35 ∼4.6 μM63 31 ± 7 (R2 = 0.9731)
anti-his tag antibody tyrosinase 35 ∼10 nM64 24 ± 5 (R2 = 0.9420)
anti-AGR2 aptamer AGR2 22 ∼13 nM60 21 ± 1 (R2 = 0.9951)
anti-protein A aptamer protein A 45 ∼0.522 μM62 14 ± 1 (R2 = 0.9177)