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. 2020 Oct 12;21(20):7517. doi: 10.3390/ijms21207517

Figure 2.

Figure 2

The cellular viability and morphology of MPP+-treated SH-SY5Y cells that were stably GPR4-overexpressing (GPR4-OE) or GPR4-knockout (GPR4-KO). 24 h serum-starved SH-SY5Y cells were treated with MPP+ (1 mM) for 24 h in serum-free culture media. (A) The morphology of SH-SY5Y GPR4-OE and GPR4-KO cells was observed through bright-field microscopy. (B) Cell viability was evaluated using an MTT assay. Mean ± SEM (n = 3) was employed to express the data. Tukey’s multiple comparison test was performed using a one-way ANOVA. Each * p < 0.05 refers to the other sample concentrations compared with the control cells.