Figure 3.

The measurement of GPR4 mRNA expression and the Bax/Bcl-2 mRNA ratio in MPP⁺- and H₂O₂-treated SH-SY5Y cells that were stably GPR4-OE or GPR4-KO. (A) An RT-PCR illustrating the mRNA expression of pro-apoptotic Bax, anti-apoptotic Bcl-2, and GAPDH in SH-SY5Y, GPR4-OE, and GPR4-KO cells after stimulation with MPP⁺ (1 mM) and H₂O₂ (125 µM) in serum-free media for 24 h. (B) A semi-quantification of the GPR4 mRNA and Bax/Bcl-2 mRNA expressions relative to GAPDH. This semi-quantification of the respective mRNA expression levels was performed on ImageJ software; GAPDH was utilised as an internal control. Mean ± SEM (n = 3) was employed to express the data. Tukey’s multiple comparison test was performed using a one-way ANOVA. Each * p < 0.05 refers to the sample concentration compared with the same group of non-treated cells.