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. 2020 Oct 19;21(20):7727. doi: 10.3390/ijms21207727

Figure 4.

Figure 4

(A) Localization of AstA-GFP or 37NtermAstA-GFP in A. nidulans strains under various growth conditions. Mitochondrial localization of AstA-GFP in hyphae grown on solid medium at 37 °C, or in cell membrane when same strain was grown in liquid minimal medium (MM) at 28 or 37 °C, or on solid medium at 28 °C (MM supplemented with 0.1 mM sulfate in all experiments). (B) Solely mitochondrial localization of hybrid 37NtermAstA-GFP protein bearing 37 N-terminal amino acids of AstA. (C) Either reactive oxygen species (ROS)-generating and glutathione (GSH)-depleting drug menadione (75 μM) or Complex III-inhibiting antimycin A (100 μM) redirects AstA to the mitochondria at a lower temperature of 28 °C. (D) Hsp70-inhibiting compound methylene blue (100 μM) or mutation of porin1 prevents mitochondrial localization of AstA at 37 °C. (right column) Scatter plots and Pearson’s coefficient of two protein signals.