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. 2020 Oct 20;21(20):7754. doi: 10.3390/ijms21207754

Figure 1.

Figure 1

Hemopexin (Hx) and lactate dehydrogenase 2 (LDH2) proteins are markers of intravascular hemolysis in the blood of wild-type and Hmox1-deficient neonatal mice. (a) Plasma levels of hemopexin (Hx) were assessed by Western blotting. The blots were reprobed with polyclonal anti-albumin antibody as a loading control. Results come from three separate blots and relative levels of proteins are shown in the graph (b). Immunolabeled Hx and albumin control bands were quantified using a Molecular Imager and the relative levels of the test proteins (means ± SD, n = 3 per group) are plotted in arbitrary units. To compare differences in plasma Hx levels between wild-type (WT) and heme oxygenase 1 (HO1) knockout (KO) groups at each time point, we used Student t-tests for all datasets; * p < 0.05. (c) Lactate dehydrogenase 2 (LDH2) plasma levels in the serum from all age groups of wild-type and Hmox1−/− mice analyzed by Western blotting (WB; representative blot, n = 3). WB PVDF membrane stained with Ponceau S was used as a protein loading control. dpp—days postpartum.