Skip to main content
. 2020 Oct 11;21(20):7483. doi: 10.3390/ijms21207483

Figure 1.

Figure 1

Figure 1

Figure 1

Resveratrol (RSV) ameliorates the impaired bone quality of mice with impaired renal function. BALB/c male mice were divided into three groups: sham + normal saline (N/S) treatment; 5/6 Nephrectomy (Nx) + N/S treatment; and 5/6 Nx + RSV treatment (n = 6 in each group) (A,B). After 4 weeks of surgery and before injection treatment, the serum levels of creatinine and blood urea nitrogen (BUN) of the 5/6 nephrectomy groups were significantly elevated compared to the sham group. (C) After 4 weeks of injections, H&E staining applied to the bone tissue showed the chondrocyte rows of the 5/6 Nx + RSV group to be more orderly than those of the 5/6 Nx + N/S group. Scale bar = 0.2 mm. (D) Immunohistochemical (IHC) staining with DAB represents the preservation of Runx2 expression (brown particles). (E) The relative interest of density (IOD) values of Runx2 in bone tissue showed that the 5/6 Nx + N/S group has the lowest expression. Scale bar = 0.2 mm. (F) The micro-CT images showed that the cancellous bone tissue and epiphyseal plate (red arrows) in the distal femur metaphysis of 5/6 Nx + RSV group were increased and thicker, respectively, compared to the 5/6 Nx + N/S group. (G) The micro-CT analysis data reveal that the bone volume over total volume (BV/TV) in the 5/6 Nx + N/S group was significantly decreased in comparison with the sham group, but the 5/6 Nx + RSV group shows an increasing trend compared to the 5/6 Nx + N/S group. (H,I) In terms of trabecular number (Tb. N) and trabecular thickness (Tb.Th), the values of the 5/6 Nx + N/S group significantly decreased compared to the sham group, and those of the 5/6 Nx + RSV group significantly increase compared to the 5/6 Nx + N/S group. (J) In terms of trabecular separation (Tb.Sp), the values for the 5/6 Nx + N/S group here are significantly increased compared to the sham group, but the 5/6 Nx + RSV group showed a decreasing trend compared to the 5/6 Nx + N/S group. Data are represented as the mean ± SEM (n = 6). * Indicates differences that are considered significant between the indicated groups (p < 0.05).