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. 2020 Oct 11;21(20):7483. doi: 10.3390/ijms21207483

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The effect of RSV on IS-induced actuate aryl hydrocarbon receptor (AhR) signaling and the rescue of osteoblast differentiation. MC3T3 E1 cells were cultured in an osteogenesis medium with IS at 0, 100, 500, and 1000 μM both with and without RSV for 2 days. (A–C) In whole cells, the protein expression of CYP1B1 significantly increased in response to 500 μM IS compared to the control group. At 1000 μM IS, the protein expression of both CYP1A1 and CYP1B1 significantly increased compared to the control group. Moreover, RSV significantly suppressed CYP1A1 and CYP1B1 expression. (D) At 500 μM IS, the expression of AHR, CYP1A1, and CYP1B1 genes was significantly increased compared to their levels in the control group. In addition, when adding 10 μM RSV to the cells already treated with 500 μM IS, the expression of AhR and CYP1B1 was significantly suppressed. (E,F) In the nucleus, the protein expression of Runx2 was significantly decreased at 500 and 1000 μM IS. (G) At 500 μM IS, the expression of RUNX2, collagen 1, alkaline phosphatase, and osteopontin genes were all significantly lower compared to their expression in the control group. Data are represented as the mean ± SEM (n = 3). * Indicates differences that were considered significant between the indicated groups (p < 0.05).

The effect of RSV on IS-induced actuate aryl hydrocarbon receptor (AhR) signaling and the rescue of osteoblast differentiation. MC3T3 E1 cells were cultured in an osteogenesis medium with IS at 0, 100, 500, and 1000 μM both with and without RSV for 2 days. (A–C) In whole cells, the protein expression of CYP1B1 significantly increased in response to 500 μM IS compared to the control group. At 1000 μM IS, the protein expression of both CYP1A1 and CYP1B1 significantly increased compared to the control group. Moreover, RSV significantly suppressed CYP1A1 and CYP1B1 expression. (D) At 500 μM IS, the expression of AHR, CYP1A1, and CYP1B1 genes was significantly increased compared to their levels in the control group. In addition, when adding 10 μM RSV to the cells already treated with 500 μM IS, the expression of AhR and CYP1B1 was significantly suppressed. (E,F) In the nucleus, the protein expression of Runx2 was significantly decreased at 500 and 1000 μM IS. (G) At 500 μM IS, the expression of RUNX2, collagen 1, alkaline phosphatase, and osteopontin genes were all significantly lower compared to their expression in the control group. Data are represented as the mean ± SEM (n = 3). * Indicates differences that were considered significant between the indicated groups (p < 0.05).