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. 2020 Oct 21;21(20):7776. doi: 10.3390/ijms21207776

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Targeted bisulfite sequencing in different endothelial cells and its precursors. Analysis of statistical significance of 16 selected regions between adult and fetal liver sinusoidal endothelial cells (a-LSECs and f-LSECs), vascular endothelial cells (vECs), mesoderm derived cells, induced pluripotent cells (iPS), erythroid progenitor cells (EPCs) and peripheral blood mononuclear cells (PBMCs). Upper panel: PCA and heatmap for significant CpGs using Anova at 5% of FDR between a-LSECs, f-LSECs, PBMCs, EPCs, iPSc, mesoderm, and vECs. Next, the line plot for mean average per CpG for every region, followed by heatmap for every region showing methylation pattern in all samples.