Table 2.
Overview of the distribution of upregulated genes compared to liquid cultures across infection stages, according to the identification of conserved domains. *: those with one candidate effector.
Functional Class | Family | 24 hpi | 48 hpi | 60 hpi | 72 hpi | 84 hpi | Total |
---|---|---|---|---|---|---|---|
Carbohydrate active enzyme | Auxiliary Activities * | 0 | 1 | 5 | 7 | 13 | 13 |
Glycoside hydrolases | 0 | 6 | 11 | 22 | 33 | 37 | |
Carbohydrate binding modules | 0 | 0 | 0 | 0 | 2 | 2 | |
Glycosyl Transferases | 0 | 0 | 1 | 3 | 6 | 6 | |
Carbohydrate Esterases | 0 | 3 | 6 | 6 | 6 | 6 | |
Polysaccharide Lyases | 0 | 0 | 1 | 1 | 1 | 1 | |
Total of CAZyme domains | 0 | 10 | 24 | 39 | 61 | 65 | |
Total of genes encoding CAZymes | 0 | 9 | 23 | 38 | 59 | 63 | |
Transcription factor | Fungal Zn(2)-Cys(6) binuclear cluster domain | 0 | 0 | 2 | 2 | 4 | 4 |
Fungal specific transcription factor | 0 | 0 | 2 | 4 | 6 | 6 | |
Helix-loop-helix DNA-binding domain | 0 | 0 | 0 | 0 | 1 | 1 | |
Zinc finger, C2H2 type | 1 | 1 | 2 | 3 | 2 | 4 | |
bZIP transcription factor | 0 | 0 | 0 | 1 | 2 | 2 | |
Spt20 family | 1 | 0 | 0 | 0 | 0 | 1 | |
CP2 transcription factor | 0 | 0 | 0 | 1 | 0 | 1 | |
Total of transcription factor domain | 2 | 1 | 6 | 11 | 15 | 19 | |
Total of genes encoding transcription factors | 2 | 1 | 4 | 9 | 12 | 16 | |
Secondary metabolism | Polyketide synthase dehydratase | 0 | 0 | 1 | 2 | 2 | 2 |
Condensation domain | 0 | 0 | 1 | 2 | 2 | 2 | |
Phosphopantetheine attachment site | 0 | 0 | 1 | 2 | 2 | 2 | |
Ketoreductase domain (KR) | 0 | 0 | 1 | 2 | 2 | 2 | |
Total of secondary metabolism domains | 0 | 0 | 4 | 8 | 8 | 8 | |
Total of secondary metabolism associated genes | 0 | 0 | 2 | 2 | 2 | 2 | |
Candidate effector | 1 | 11 | 19 | 26 | 24 | 27 | |
Peptidase | Serine peptidase | 1 | 2 | 3 | 6 | 9 | 10 |
Metallopeptidase | 0 | 1 | 2 | 3 | 6 | 6 | |
Cystein peptidase | 2 | 1 | 1 | 0 | 3 | 3 | |
Peptidase inhibitor | 0 | 0 | 0 | 0 | 1 | 1 | |
Total of genes encoding peptidases | 3 | 4 | 6 | 9 | 19 | 20 | |
Transmembrane transporter | The Ca2+:Cation Antiporter (CaCA) Family | 0 | 0 | 1 | 4 | 0 | 4 |
The Amino Acid/Auxin Permease (AAAP) Family | 0 | 0 | 0 | 3 | 2 | 3 | |
The ATP-binding Cassette (ABC) Superfamily | 0 | 1 | 2 | 4 | 4 | 5 | |
The Major Facilitator Superfamily (MFS) | 0 | 0 | 3 | 7 | 13 | 14 | |
The Amino Acid-Polyamine-Organocation (APC) Family | 0 | 0 | 3 | 2 | 3 | 4 | |
The Proton-dependent Oligopeptide Transporter (POT) Family | 0 | 0 | 0 | 0 | 1 | 1 | |
The Small Conductance Mechanosensitive Ion Channel (MscS) Family | 0 | 0 | 0 | 1 | 1 | 1 | |
The P-type ATPase (P-ATPase) Superfamily | 0 | 0 | 1 | 3 | 2 | 4 | |
The Mitochondrial Carrier (MC) Family | 0 | 0 | 1 | 1 | 1 | 1 | |
The Oligopeptide Transporter (OPT) Family | 0 | 0 | 0 | 0 | 1 | 1 | |
The Transient Receptor Potential Ca2+ Channel (TRP-CC) Family | 0 | 0 | 0 | 1 | 1 | 1 | |
Threonine/serine exporter | 0 | 0 | 0 | 1 | 1 | 1 | |
Mitochondrial calcium uniporter | 0 | 0 | 0 | 0 | 1 | 1 | |
Total of transmembrane transporter genes | 0 | 1 | 11 | 27 | 31 | 41 | |
Other pathogenicity related-factors | Nudix | 0 | 0 | 1 | 1 | 2 | 2 |
Necrosis and ethylene-inducing peptide * | 0 | 3 | 6 | 6 | 6 | 6 | |
Stress response | 0 | 0 | 3 | 12 | 13 | 16 | |
Pathogenicity | 0 | 2 | 3 | 4 | 8 | 9 | |
Oxydoreduction | Cytochrome P450 | 0 | 0 | 2 | 7 | 10 | 11 |
NAD/FAD binding and other oxydo-reductase | 3 | 8 | 17 | 37 | 53 | 58 | |
Cellular process | AAA family | 0 | 0 | 0 | 0 | 2 | 2 |
Binding | 6 | 7 | 14 | 15 | 10 | 24 | |
Cell differentiation | 1 | 1 | 1 | 1 | 0 | 1 | |
DNA repair | 1 | 1 | 1 | 1 | 1 | 1 | |
Intracellular transport | 1 | 1 | 3 | 10 | 8 | 13 | |
Methyltransferase | 1 | 1 | 3 | 6 | 10 | 13 | |
Mitochondria | 1 | 1 | 2 | 2 | 1 | 2 | |
Protein kinase | 1 | 1 | 2 | 3 | 6 | 7 | |
Transcription | 4 | 3 | 3 | 6 | 4 | 8 | |
Translation | 3 | 5 | 9 | 10 | 5 | 12 | |
Ubiquitinilation | 1 | 1 | 1 | 2 | 4 | 4 | |
Metabolic process | Alpha/beta hydrolase | 0 | 0 | 1 | 1 | 2 | 2 |
AMP-binding enzyme | 0 | 0 | 1 | 1 | 2 | 2 | |
Carbohydrate metabolism | 1 | 1 | 2 | 2 | 6 | 6 | |
Coenzyme A metabolism | 0 | 0 | 0 | 0 | 1 | 1 | |
Lipid metabolism | 0 | 0 | 0 | 4 | 5 | 6 | |
Cell structure | 4 | 4 | 6 | 9 | 6 | 11 | |
Hypothetical protein | 27 | 33 | 60 | 93 | 120 | 144 | |
Others | 2 | 3 | 18 | 40 | 51 | 63 |