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. 2020 Oct 21;12(10):1202. doi: 10.3390/v12101202

Figure 1.

Figure 1

The three-domain structure of integrase from HIV-1, and the constructs of IN used in this study. (A): The complete genome of HIV-1 is shown. Integrase (IN) is encoded in the very C-terminal region of the Pol domain from the GagPol polyprotein precursor. The schematic view and three-dimensional (3D) representation of IN are shown. The domain structure of IN is formed by the N-terminal domain (NTD, in green) and the C-terminal domain (CTD, in cyan) connected to the catalytic core domain (CCD, in red). The second CCD of the IN dimer visible in the cryo-EM structure is shown in grey [22]. Full-length integrase with a C-terminal His-tag (IN-H6), with a deletion of the NTD (IN-∆N), or with a deletion of the NTD and the CTD (IN-CCD) were constructed. (B) Constructs of the CTD on IN starting at residues 213 (IN-CTD213) or 222 (IN-CTD222) are shown. (C) Constructs of IN-CTD222 with a deletion of 5, 10, or 15 C-terminal residues. These derivatives of IN were tested for their association with mLysRS (Kd determined as reported in Figure 2 are indicated; values are the means ± SEM of three independent experiments).