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. 2020 Oct 21;12(10):1202. doi: 10.3390/v12101202

Figure 2.

Figure 2

Association of the derivatives of IN with mLysRS. The binding affinities of mLysRS to IN constructs were determined in an HTRF assay using 1.5 nM of HA-tagged mLysRS and increasing concentrations of His-tagged IN derivatives, expressed as dimer (IN, IN-∆N, IN-CCD) or monomer (CTD213, CTD222, CTD222∆C5, CTD222∆C10, CTD222∆C15) concentrations. Experimental values (symbols) were fit (curves) to a binding equation assuming that one molecule of IN binds one molecule of mLysRS. The binding constants and the associated standard deviations (n = 3) are indicated in Figure 1.