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. 2020 Oct 21;12(10):1202. doi: 10.3390/v12101202

Figure 5.

Figure 5

Cross-linking of the pBpa-containing mLysRS variants with IN-CTD222. Wild-type mLysRS (WT) or mLysRS containing pBpa inserted at different positions (D222 to E576) were incubated at a dimer concentration of 85 nM with IN-CTD222 (monomer concentration of 1.12 µM) and exposed to UV light at 365 nm for 70 min. Samples recovered before (A) or after (B) exposure to UV were analyzed by SDS-PAGE and western blotting using anti-IN-CTD antibodies. The lanes containing the stained size-marker are added on the left (in kDa). The migration level corresponding to an expected cross-linked polypeptide (79 kDa) containing one monomer of IN-CTD222 and one monomer of mLysRS is indicated by a black arrowhead. This experiment is the representative of two independent experiments.