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TUNEL assay. Compound 3 induces apoptotic cell death. TUNEL staining (green) in MCF-7 (A) and SkBr3 (C) cells treated for 24 h with vehicle or 10 μM 3 (compound 3), as indicated. Nuclei were stained by DAPI (blue). Each experiment shown is representative of 20 random fields observed. (B,D) Percentages of TUNEL-positive cells over vehicle-treated cells, (◦) indicates p < 0.05 for cells receiving vehicle versus treatments. Scale bar 200 μm.
