Cellular density separation of whole blood following a two-spin centrifugation procedure using the PurePRP-SP® device (EmCyte Corporation, Fort Myers, FL, USA). After the first centrifugation procedure, the whole blood components are separated in two basic layers, the platelet (poor) plasma suspension and the RBC layer. In A, the second centrifugation step has been completed. The factual needed PRP volume can be extracted for patient application. The magnification in B shows at the bottom of the device the organized multicomponent buffy coat layer (indicated by the blue lines), containing high concentrations of platelets, monocytes, lymphocytes, based on density gradients. In this example, a minimal percentage of neutrophils (<0.3%) and RBCs (<0.1%) will be extracted, following a neutrophil poor C-PRP preparation protocol.