Table 1.

Kinetic parameters of the mutations in the DPN–triloop interaction.

	KM (mM)	kcat (s−1)	kcat/KM (s−1·M−1)
WT	0.64 ± 0.03	0.65 ± 0.01	1.0 × 103
D57A	5.6 ± 0.7	0.025 ± 0.001	4.5
D57N	0.83 ± 0.07	0.021 ± 0.0005	25
S93A	12 ± 2	0.045 ± 0.003	3.8
S93N	8.8 ± 0.5	0.057 ± 0.001	6.5
N128A	26 ± 3	0.26 ± 0.01	10
N128D	21 ± 2	0.041 ± 0.002	2.0