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. 2020 Oct 15;21(20):7627. doi: 10.3390/ijms21207627

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Proliferation and colony-forming ability after Sox9 knockout and knockdown in HTB94 chondrosarcoma cells. (A) Representative Western blot images of SOX9 after siRNA-mediated SOX9 knockdown and Crispr/Cas9-mediated SOX9 knockout (B). (C,D) SOX9 knockdown and knockout cells revealed an increase in doubling time and (E,F) a decrease in the ability to form colonies from single cells cultured in agarose. (G,H) Representative images of colony formation after SOX9 knockdown and knockout (Magnification 10×). Results are the means ± SD; * p ≤ 0.05.