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. 2020 Oct 19;21(20):7737. doi: 10.3390/ijms21207737

Figure 1.

Figure 1

Autocrine heregulin up-regulates IL-8 in luminal breast cancer cells. (A) Left. Schematic representation of the structural heregulin (HRG) mutants employed. Right. Forty-eight-hour conditioned media from MCF-7/pBABE, MCF-7/HRG, MCF-7/HRG-M1, and MCF-7/HRG-M4 cells were assayed for cytokine content as described in the “Materials and methods” section. Shown are representative results (n = 3) revealing conspicuous changes in uPAR, amphiregulin, and IL-8 secreted from MCF-7/HRG cells as compared with MCF-7/pBABE control counterparts. (B) IL-8 concentration in conditioned media from MCF-7/pBABE, MCF-7/HRG, MCF-7/HRG-M1, and MCF-7/HRG-M4 cells was assessed by ELISA. Values represent mean (columns) ± S.D. (bars) from three independent experiments. (** p < 0.005; n.s. not statistically significant).