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Nuclear Overhauser effect spectroscopy (NOESY) spectrum of 4″-aminopropyl azithromycin in (a) acetonitrile-d3 prior to addition of the E. coli ribosome compared with (b) NOESY (red and pink signals) and transferred nuclear Overhauser effect spectroscopy (trNOESY) spectrum (blue and green signals) of the same compound in Tris-d11 buffer (pH 7.4) prior to and after addition of the E. coli ribosome, respectively. The spectra were recorded at 25 °C and 600 MHz.
