. 2020 Oct 13;21(20):7553. doi: 10.3390/ijms21207553

Table 2.

Kinetic parameters for the catalytic activity of the hemin and hemin-peptide complexes in the oxidation of ABTS by hydrogen peroxide, in phosphate buffer, pH 7.4, at 25 °C.

Complex	k₁ (M⁻¹s⁻¹)	K_M (mM)	k_cat (s⁻¹)	k_cat/K_M (M⁻¹s⁻¹)
Hemin	42 ± 4	1.90 ± 0.20	0.011 ± 0.001	6
Hemin-PrP_106–114	51 ± 5	4.70 ± 0.40 *	0.068 ± 0.002 *	14 *
Hemin-PrP_95–114	62 ± 1 *	2.24 ± 0.18	0.048 ± 0.012 *	22 *
Hemin-PrP_84–114	75 ± 4 *	1.59 ± 0.15	0.054 ± 0.001 *	34 *
Hemin-PrP_76–114	54 ± 1 *	0.94 ± 0.17 *	0.056 ± 0.002 *	60 *
Hemin-AcR1τ ^a	73 ± 5	1.90 ± 0.10	0.080 ± 0.002 *	43
Hemin-Aβ16 ^a	43 ± 5	0.05 ± 0.05	0.070 ± 0.002	134
HRP ^b	10⁷	0.64	45.5	7.1 × 10⁴

^a Data from Pirota et al. [43]. ^b Data from Dunford [55]. * parameter significantly different from that of hemin (T-test, p = 0.05, n = 3).