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Lcn-2 is expressed from different sources during CLP-induced renal injury progression. (a) Schematic representation for the isolation of both macrophages (MΦ) and tubular epithelial cells (TEC). (b–d) ELISA of Lcn-2 protein expression in the supernatant of short-term cultured and freshly isolated (b) peritoneal MΦ, (c) TEC, and (d) renal MΦ. n = 6 isolated primary peritoneal MΦ, TEC, and renal MΦ per group, *** p < 0.001; One-way ANOVA followed by Tukey’s multiple comparison test.
