Figure 5.

Renal recovery correlates with both total iron as well as Lcn-2-bound iron from renal MΦ. mRNA expression of (a) PCNA and (b) Stathmin relative to the housekeeping gene RPS27a. n = 5 animals per group. ** p < 0.01, *** p < 0.001; one-way ANOVA followed by Tukey’s multiple comparison test. (c–h) Correlation between (c,e,g) PCNA or (d,f,h) Stathmin with either (c,d) Lcn-2 protein expression, (e,f) Lcn-2-bound iron, or (g,h) total iron measured in the supernatant of renal MΦ at 48 h after CLP treatment (all values from this time-point were included; R² and p-values are depicted in the individual graph).