Figure 7.

Knockdown of FHL2 inhibited EOC tumorigenesis in vivo. (A) SKOV-3 cells were transfected by either lentivirus-based non-targeting (Ctrl) or FHL2 shRNA vectors (shFHL2), and stable cell lines were constructed. Western blot results showed FHL2 expression in control (Ctrl) and FHL2 knockdown stable cells (shFHL2). Beta actin was used as loading control. (B) SKOV-3 cells transfected with lentivirus-based non-targeting or FHL2 knockdown vector (shFHL2) were implanted subcutaneously into the right and left shoulders of athymic nude mice, respectively. Images showed the tumor formation in the right shoulders of nude mice (Top panel). The formed tumors from control were isolated and presented (lower panel). No xenograft was found in FHL2 knockdown group. The minimum scale of the ruler is mm. (C) Growth curve showed the xenografts derived from control and FHL2 knockdown cells (shFHL2). Each point represents mean tumor volume ± S.E.M. of 5 mice. (D) Weights of tumor xenografts derived from control and FHL2 knockdown group (shFHL2). Each bar represents mean ± S.E.M.3.