Figure 2.

MHCII⁺ tAMs subpopulation shows signs of polynucleation upon allergic asthma (A) CD11c⁺SiglecF⁺MHCII⁺ resident tAMs from HDM-triggered asthmatic lungs but not the MCHII- subpopulation; showing cells with four to six nuclei. Data shown are representative of at least three independent experiments. Pictures were taken with a 20× objective. (B) CD11c⁺ SiglecF⁺ tAMs from HDM-induced asthma show increased DNA content by Hoechst 33342 staining. Data are representative of at least two independent experiments. (C) DNA content increase is observed in tissue tAMs but not in bronchoalveolar macrophages as shown by Hoechst 33342 staining. Data are representative of at least two independent experiments. (D) Histology section of HDM-treated mouse. Arrows indicate the presence of polynucleated cells in the alveolar compartment. Pictures were taken using a 20× objective.