Figure 1.

Role of myeloid cell ezrin in adhesion to endothelial cells and transmigration. (A) Immunoblot of lysates from THP-1 cells subjected to lentivirus-mediated stable knockdown with shRNA targeting ezrin (ShEZR) or control shRNA (ShCtrl). (B) Calcein-AM-labeled ShCtrl and ShEZR THP-1 cells were incubated with human umbilical vein endothelial cells (HUVEC) for 3 h and cell adhesion was determined by immunofluorescence imaging (left) and by the quantification of fluorescence intensity (right). (C) The chemoattraction of calcein-AM-labeled ShCtrl and ShEZR THP-1 cells towards conditioned medium (CM) from breast cancer cell lines was determined by a trans-well migration assay. Mean ± standard deviation; * and ** indicate p < 0.05 and 0.01, respectively.