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. 2020 Oct 21;9(10):2332. doi: 10.3390/cells9102332

Figure 1.

Figure 1

Characteristics of RJK and RJKEB cells. (A) RJK cells, live imaging, Ob: 10×; (B) RJKEB cells, live imaging, Ob: 10×; (C) RJK and RJKEB growth curves. To plot cell growth curves, the number of RJK and RJKEB cells was counted using FACS analysis for 4 days. The mean ± SD of three independent experiments (** p ˂ 0.01); (D) standard RJK karyotype. 2, 3, 8—normal chromosomes. Z1-Z15—marker chromosomes. n = 20; (E) RJK karyotype with loss of chromosome Z10, n = 18; (F) karyotype with centromeric break in chromosome 2, del p terZ8, chromosome Z15 is absent. n = 18; (G) karyotype of RJKEB cells. A cell variant with two copies of the Z6 chromosome labeled with HSR of different lengths. Chromosomes Z11 and Z15 are absent. n = 18. Ob: 100×. Abbreviations: RJK—Chinese hamster cells CHLV-79 RJK; RJKEB—RJK resistant to ethidium bromide; FACS—fluorescence-actevated cell sorting; del—deletion; p—short chromosome arm; q—long chromosome arm; n—number of chromosomes in the karyotype; HSR—homogeneously stained region.