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. 2020 Oct 21;9(10):2332. doi: 10.3390/cells9102332

Figure 3.

Figure 3

Changes in the karyotype of RJK and RJKEB cells survived after exposure to PAA. (A) Aneuploid RJK-PAA karyotype with 3 copies of chromosome 2; chromosomes—3, Z1, Z4, Z5, Z9, Z12—2 copies; lack of chromosomes Z2, Z3, Z11; del 2p ter (in one copy) and del Z7 pter (in one copy), del 3p (in one copy, tr 3p: 3p ter (in another copy). n = 23. (B) Aneupolyploid RJK-PAA karyotype, chromosome Z2 is missing; centromeric breaks in one copy of chromosome Z1 (p-arm, q-arm, in one copy); del 2p ter (in one copy) and delZ5qter (in two copies). n = 44. (C) Aneuploid RJKEB-PAA karyotype with impaired copying of chromosomes Z1, Z5, Z6; lack of chromosomes Z2, Z8; centromeric breaks in Z3 chromosome (p-arm, q-arm), the presence of HSR of different lengths in Z6. n = 20. (D) Aneuploid RJKEB-PAA karyotype with impaired copy number of chromosome 2 (3 copies), lack of chromosomes Z2, Z14, Z15; del 2q—ter (in one copy), break in Z1 pter. n = 17. Abbreviations: RJK—Chinese hamster cells CHLV-79 RJK; RJKEB—RJK resistant to ethidium bromide; PAA—polyallylamine hydrochloride; RJK-PAA—RJK cells after PAA treatment; RJKEB-PAA—RJKEB cells after PAA treatment; EB—ethidium bromide; del-deletion; ter-terminal end of the chromosome; p—short arm of the chromosome; q—long arm of the chromosome; n—number of chromosomes in the karyotype; HSR—homogeneously stained region.