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. 2020 Oct 21;9(10):2332. doi: 10.3390/cells9102332

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Viability of RJK, RJK-PAA, RJKEB, RJKEB-PAA cells after treatment with various doses of DOX. (A) Viability of RJK, RJK-PAA, RJKEB and RJKEB-PAA treated with DOX for 24 h. MTT assay. (B) The cloning efficiency of RJK, RJK-PAA, RJKEB, RJKEB-PAA after DOX treatment. (C) Petri dishes with colonies of RJK, RJK-PAA, RJKEB, RJKEB-PAA cells stained with crystal violet dye. (D) Quantitation of colonies in RJK, RJK-PAA, RJKEB, RJKEB-PAA cultures after DOX treatment. The mean ± SD of three independent experiments are presented (** p ˂ 0.01). Abbreviations: RJK—Chinese hamster cells CHLV-79 RJK; RJKEB—RJK resistant to ethidium bromide; PAA—polyallylamine hydrochloride; RJK-PAA—RJK cells after PAA treatment; RJKEB-PAA—RJKEB cells after PAA treatment; EB—ethidium bromide; DOX—doxorubicin.