Table 1.

The primers and conditions for qRT-PCR.

Symbol	Primer Sequence	Amplification Conditions	PCR Product Size (bp)	NCBI Reference Sequence
p53	F 5′ GTTGGCTCTGACTGTACCAC 3′ R 5′ AGGGTGAAATATTCTCCATC 3′	93 °C, 20 s, 57 °C, 20 s, 72 °C 30 s	317	NM_001243976.1
topo2-α	F 5′ GGGAGACTCAGCCAAAACAC 3′ R 5′ CAGCATCATCTTCAGGTCCA 3′	93 °C, 20 s, 58 °C, 20 s, 72 °C 30 s	560	NM_001246738.1
mdr1	F 5′ ATCGACGGTCAGGACATCAG 3′ R 5′ TTCAGCGATAGTGGTGGCAA 3′	93 °C, 20 s, 60 °C, 20 s, 72 °C 30 s	102	XM_027439202.1
c-fos	F 5′ GCAGCCAAATGCTGGAATCG 3′ R 5′ CCAGTGATGTCTTGGGCTCA 3′	93 °C, 20 s, 60 °C, 20 s, 72 °C 30 s	310	NM_001246683.1
grp78	F 5′ GATGCGGCCAAGAACCAGCT 3′ R 5′ CGCATGACATTCAGTCCAGC 3′	93 °C, 20 s, 63 °C, 20 s, 72 °C 30 s	359	NM_001246739.2
hsp90	F 5′ AATCGGAAGAAGCTTTCAGA 3′ R 5′ GTGCTTGTGACAATACAGCA 3′	93 °C, 20 s,56 °C, 20 s, 72 °C 30 s	257	NM_001246821.1
hsc70	F 5′ ATCCCCAAGATTCAGAAGCT 3′ R 5′ TTGATGAGGACAGTCATGAC 3′	93 °C, 20 s, 56 °C, 20 s, 72 °C 30 s	218	NM_001246729.1
actin	F 5′ GCTGAGAGGGAAATTGTGCGTG 3′ R 5′ CGGTGGACGATGGAGGGGCCG 3′	93 °C, 20 s, 68 °C, 20 s, 72 °C 30 s	506	XM_007648665.3

The primers and conditions for qRT-PCR. Abbreviations: qRT-PCR—quantitative reverse transcription polymerase chain reaction; F—forward; R—reverse; p53—tumor protein p53 (Tp53); topo 2-α—DNA topoisomerase 2; mdr1—multidrug resistance 1; ATP binding cassette subfamily B member 1 (Abcb1); c-fos—Fos proto-oncogene; grp78—heat shock protein family A (Hsp70) member 5; hsp90—heat shock protein 90 alpha family class A member 1 (Hsp90aa1); hsc70—heat shock protein family A (Hsp70) member 8 (Hspa8); actin—actin beta (Actb).