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. 2020 Oct 12;21(20):7499. doi: 10.3390/ijms21207499

Figure 4.

Figure 4

Detection of transgenic plants. (A) Diagram of the 35S:TaGAPC2-6D constructs. TaGApC2-6D CDS was fused with the GFP-coding region driven by a 35S promoter. The primers pr1, pr2, and pr3, used to analyze TaGApC2-6D and TaGApCs-GFp in transgenic Arabidopsis plants, are shown. (B) PCR analysis of TaGApC2-6D over-expressing transgenic Arabidopsis using primer pairs of pr1 and pr2. (C) PCR analysis of TaGApC2-6D over-expressing transgenic Arabidopsis using primer pairs of pr1 and pr3. (D) Quantitative real-time PCR (qRT-PCR) validation of TaGApC2-6D in the aboveground part of the three-week-old Arabidopsis plant. The AtTubulin gene was used as an internal reference (*** p < 0.001). (E) The enzyme activities of glyceraldehyde-3-P dehydrogenase (GAPDH) in the aboveground part of the three-week-old Arabidopsis plant (** p < 0.01).