Table 2.

Apparent melting temperatures (T_1/2) of GROs in K⁺ and PBS buffers at different DNA concentration and heating rate determined by circular dichroism (CD) melting curves.

	T1/2 (°C) 1 in K+ Buffer				T1/2 (°C) 1 in PBS Buffer
	1 µM		20 µM		1 µM		20 µM
	0.5 °C/min	2 °C/min	0.5 °C/min	2 °C/min	0.5 °C/min	2 °C/min	0.5 °C/min	2 °C/min
AS1411	48	54	50	55	38	39	41	44
AS1411-G	n.d.	n.d.	n.d.	n.d.	86	86	93	93
AS1411-GT	72	79	76	85	60	60	64	66
AS1411-GT-T8	70	72	92	93	65	67	88	90
AS1411-GT-5′tr	59	60	59	61	51	52	51	54

¹ Errors were ± 1 °C. n.d. = not determinable (T_1/2 > 93 °C).