Figure 5.

Gene expression of TMRM-low and high cells in basal condition. mRNA expression of markers associated to undifferentiated cells (A) and lineage specific cells (B) were determined by qRT-PCR. MDR-1: multidrug resistance 1; POU5F1: POU domain, class 5, transcription factor 1; NANOG1: homeobox protein NANOG; KLF4: Krüppel-like factor 4; NKX2.5: NK2 homeobox 5; TBX5: T-Box transcription factor 5; PPARγ: peroxisome proliferator-activated receptor gamma; RUNX-2: runt-related transcription factor 2; KDR: kinase insert domain receptor; ACTA2: actin alpha 2, smooth muscle; SOX9: SRY-box transcription factor 9. Data are represented as mean ± SD of the fold change. n = 4/5 per group. Statistical differences were calculated significant as * p < 0.05, determined by paired Student’s t-test.