Figure 7.

In vitro cardiac differentiation of TMRM-low and high cells. Expression of early cardiac gene/protein: T-Box transcription factor 5 (TBX5) and late cardiac genes cTNT (TNNT2) and α-sarcomeric actin (ACTC1) in TMRM-low and high cells after 1 week of culture in cardiomyogenic differentiation media. The expression of these genes was analyzed by qRT-PCR (n = 5) (A), and Western blot (n = 4) (B) Data are represented as mean ± SD of the fold change. Statistical differences were calculated significant as * p < 0.05, determined by paired Student’s t-test.