Figure 8.

In vitro adipogenic differentiation of TMRM-low and high cells. (A) The culture in pro-adipogenic conditions for 1 week favored morphological differentiation in particular of TMRM-high cells, as evidenced by accumulation of lipid droplets in the cytoplasm, stained by Oil-Red-O (left panels). The bar graph shows the relative quantification (right panel). (B) Expression of the pro-adipogenic genes PPARγ: peroxisome proliferator-activated receptor gamma, PLIN1: perilipin 1, and FABP4: fatty acid-binding protein 4. Data are represented as mean ± SD of the fold change. n = 4 per group. Statistical differences were calculated significant as * p < 0.05, determined by Student’s t-test.