Figure 12.

TMRM transition in culture and during differentiation. (A) Fluorescence levels of TMRM in the hCmPC population: before sorting (green histogram) and after selection to separate the population with the highest levels of TMRM (high, light grey histogram) from the population expressing the lowest levels of TMRM (low, black histogram). (B) Histogram plot of the transition level of TMRM expression in TMRM-low population in a time course experiment from T0 to T7 and its relative quantification (C). (D,E) Histogram plot and quantification of TMRM expression levels shown as MFI in TMRM-low and high population after endothelial (D) and cardiomyogenic (E) differentiation. Data are represented as mean ± SD. n = 3 per group. Statistical differences were calculated significant as * p < 0.05, determined by Student’s t-test.