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. Author manuscript; available in PMC: 2021 Sep 29.
Published in final edited form as: Biochemistry. 2020 Sep 18;59(38):3554–3561. doi: 10.1021/acs.biochem.0c00719

Figure 4. Disappearance but not formation of RAD-51 foci is delayed by rev-1 mutation after ICL induction in mitotically proliferating germ cells.

Figure 4.

(A and B) L4 stage worms of wild type (N2) and xpa-1(RNAi), rev-1(tm8701) and rev-1(tm8701); xpa-1(RNAi) were treated with TMP (200 μM) for 40 min and exposed to UV-A light (100 J/m2). Gonads were dissected, fixed, and immunostained at 12 and 24 h using RAD-51 antibody. Scale bar, 10 μm. (C) The number of RAD-51 foci per nuclear focal plane was scored for over 100 mitotic germ cells in each stain. P values: *, P<0.05; **, P<0.01; ***, P<0.001; ns, no statistical significance. See Table S2 for One-way ANOVA analysis.