FIGURE 2.
Strategies for creation and selection of sdAbs libraries. sdAb diversity is obtained from B cells of naïve or immunized animal, or from synthetic DNA library. Expression of single sequences results in the presence of a unique sdAb variant at the surface of each individual host (typically phages, bacteria, or yeast cells). Multiple rounds of selective pressure are applied to the host, resulting in the identification of strong antigen binders. Adapted from Hoogenboom 37 . sdAbs, single‐domain antibodies