FIGURE 1.

Western blot analysis of endogenous tyrosyl‐tRNA synthetase (YRS) in circulating plasma and platelets. (A) Plasma samples collected from healthy donors were analyzed by SDS‐PAGE under non reduced conditions and blotted with anti‐YRS monoclonal antibody (A‐10). Recombinant YRS^WT was loaded as a positive control. The asterisk represents oligomerized YRS and the arrowhead points to the truncated form of YRS. In the lower panel, the same human plasma samples (N1‐N3) were analyzed by SDS‐PAGE under reduced conditions and blotted with the same antibody (A‐10). The arrow indicates full‐length YRS. (B) Platelet lysates obtained from healthy human donors and C57BL/6J mice were loaded onto SDS‐PAGE, together with whole cell lysates from B16 melanoma cell line and RAW cells. The arrow corresponds with full‐length YRS. The arrowhead represents the truncated form of YRS