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. 2020 Sep 14;4(7):1167–1177. doi: 10.1002/rth2.12429

FIGURE 2.

FIGURE 2

YRS can be cleaved by matrix metalloproteinase (MMP) and elastase present in plasma. (A) Schematic representation of YRS and its various forms. YRSMini and YRSMini+ are truncated, active forms. (B) Recombinant dual‐tagged YRS (dt‐YRS) was incubated in plasma with or without inhibitors against elastase, MMP, or both for 1 h. Samples were analyzed by Western blotting and the band intensity was quantified using LI‐COR ImageStudio. The intensity of recombinant dt‐YRS that was promptly denatured without incubation was used as a reference for normalization. The experiment was repeated 4 times using plasma derived from different donors, and the mean value with standard deviation is shown. *P < .05 by one‐way ANOVA with Dunn’s multiple comparison test