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. 2020 Oct 14;20(6):362. doi: 10.3892/ol.2020.12226

Figure 6.

Figure 6.

Invasion is suppressed in MSTO-211H cells with reduced Bex1 expression. The degree of invasion in MSTO-211H cells was compared using the DL-CGH assay and comparisons were made between the following cells: Non-treated, control-treated (only Lipofectamine), control non-silencing siRNA-treated, and Bex1-siRNA-treated. (A) Comparison using DL-CGH of phase difference capture at day 0 and day 14. (B) Enlarged images of boundary between the inner layer and the outer layer. MSTO-211H with Bex1 knocked down showed almost no cells with dendriform shape. (C) Comparison using DL-CGH of living cells stained with neutral red at day 0 and day 14. (D) Histogram showed the mean and standard deviation of numbers of red-stained pixels of each DL-CGH sample at day 10. The pixel numbers of red-stained areas were 220,502 in normal (non-treated), 236,713 in control-treated (only Lipofectamine), 205,433 in control non-silencing siRNA-treated, and 128,262 in Bex1-siRNA-treated. The pixel numbers of Bex1 RNAi were also significantly decreased. Bex1, brain-expressed X-linked protein 1; siRNA, small interfering RNA; DL-CGH, double-layered collagen gel hemispheres.