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. 2020 Oct 14;20(6):359. doi: 10.3892/ol.2020.12223

Figure 2.

Figure 2.

Overexpression of miR-454-3p suppresses the proliferation and promotes the apoptosis of ESCA cells in vitro. (A) Reverse transcription-quantitative PCR was used to analyze the relative expression levels of miR-454-3p in EC9706 and TE-1 cells transfected with miR-454-3p mimics/miR-NC as well as in ECA109 and TE-8 cells transfected with miR-454-3p inh/NC inhibitors (NC inh). (B) A Cell Counting Kit-8 assay was used to evaluate the proliferation of EC9706, TE-1, ECA109 and TE-8 cells transfected with miR-454-3p plasmid/NC mimics or miR-454 inhs/NC inh for 1, 2, 3 and 4 days. (C) Colony formation assays demonstrated the effect of miR-454-3p on the proliferation of EC9706, TE-1, ECA109 and TE-8 cells transfected with miR-454-3p mimics/miR-NC or miR-454-3p inh/NC inh and the colony number was quantified after transfection. (D) The apoptotic EC9706, TE-1, ECA109 and TE-8 cells transfected with miR-454-3p mimics/miR-NC or miR-454-3p inh/NC inh were determined using flow cytometry analysis. Results are presented as mean ± SD. *P<0.05, **P<0.01 and ***P<0.001 vs. respective control. miR, microRNA; ESCA, esophageal cancer; NC, negative control; inh, inhibitor; miR-NC, negative control mimic.