Fig. 2.

Prediction and detection of miR-205 targets. a miR-205 binding sites in the 3′UTR of CHN1 in cross-species. b Confirmation of the miR-205 target gene. HeLa cells were cotransfected with negative control (NC), miR-205 mimic, and pmirGLO-CHN1-wt for dual luciferase assays. PRL-TK, containing Renilla luciferase, was cotransfected with the 3′UTR of CHN1 for data normalisation. c Mutation of the miR-205 binding site. HeLa cells were cotransfected with NC, miR-205 mimic, and pmirGLO-CHN1-mt for dual luciferase assays. *P < 0.05