Table 2.
The sequences of siRNA, miRNA and primer used in this study
| Gene | Sequence |
|---|---|
| CHN1 3’UTR | Forward: 5′-(C)TTGAGGGGAAAAGAAATG-3’ |
| Reverse: 5′-ATGTAACAGCCAGAGGTGC-3’ | |
| CHN1-mut | Forward: 5′-AGAAATGTTTTACAGGCTGGCCGATGTTTTATAG-3’ |
| Reverse: 5′-CGGCCAGCCTGTAAAACATTTCTTTTCCCCTCA-3’ | |
| Has-miR-205 | RT-205: 5′-GTCGT ATCCA GTGCA GGGTC CGAGG TATTC GCACT GGATA CGACC AGACT-3’ |
| Forward: 5′-AATTGTCCTTCATTCCACCGG-3’ | |
| Reverse: 5′-GTGCAGGGTCCGAGGT-3’ | |
| Human U6 | Forward: 5′-CGCTTCGGCAGCACATATAC-3’ |
| Reverse: 5′-TTCACGAATTTGCGTGTCAT-3’ | |
| CHN1 | Forward: 5′-GGAGCTACCTCATCCGGGAG-3’ |
| Reverse: 5′-TGTGTCTCTTTCAGGACTGGCA-3’ | |
| GAPDH | Forward: 5′-GGTCTTACTCCTTGG AGGCCATGTG-3’ |
| Reverse: 5′-ACCTAACTACATCGTTTACATGTT-3’ | |
| LNA-miR-205 probe | 5′-CAG(+A)C(+T)CCGG(+T)GGAA(+T)GA(+A)GGA-Dig-3’ |
| Hsa-miR-205 | 5′-UCCUUCAUUCCACCGGAGUCUG-3’ |
| Hsa-miR-205 mimic | 5′-GACUCCGGUGGAAUGAAGGAUU-3’ |
| MircoRNA inhibitor NC | 5′-CAGUACUUUUGUGUAGUACAA-3’ |
| Hsa-miR-205 inhibitor | 5′-CAGACUCCGGUGGAAUGAAGGA-3’ |
| Negative control | 5′-UUCUCCGAACGUGUCACGUTT-3’ |
| si-CHN1 | 5′-GGCUUGAUUACUCUCUAUATT-3’ |