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. 2020 Oct 13;11:570296. doi: 10.3389/fimmu.2020.570296

Figure 2.

Figure 2

Histological and immunohistochemical analyses of the small intestine. Hematoxylin-eosin staining of the duodenum (A), jejunum (D), and ileum (G). and immunohistochemical analysis of Crp1 in the duodenum (B), the jejunum (E), and the ileum (H) and Crp4 in the duodenum (C), the jejunum (F), and the ileum (I) of mouse small intestine. Immunohistochemical analyses of isolated single crypt from duodenum (J), jejunum (K), and ileum (L) using confocal microscopy. Representative images of each site with Crp1 and lysozyme staining together with 4’,6-diamidino-2-phenylindole (DAPI), F-actin, and differential interference contrast (DIC) were shown. The same crypt observed in (J–L) with Crp1, lysozyme, and F-actin were shown by 3D images in (M–O), respectively. A representative image of 10 crypts was shown.