Fig. 2.

Downregulation of JARID1B expression led to decrease CRC proliferation in vitro and in vivo. a, b Western blot and qRT-PCR analysis of JARID1B protein expression in human nonmalignant cell line and CRC cell lines. c, d, e Cells proliferation capacities were detected by EdU, colony formation assay and RTCA assays in CRC DLD-1-cells transfected with the shJARID1B#1/#2 plasmid. f In-vivo tumor formation was examined by subcutaneously injecting CRC DLD-1-shNC (left) or CRC DLD-1-shJARID1B#1 (right) cells into the flanks of nude mice. Representative images obtained by an IVIS in-vivo imaging station after inoculation were shown. Corresponding tumor growth curves were obtained (g). h Tumor weight was counted at 40 days, JARID1B knockdown inhibited tumor proliferation.*p < 0.05, **p < 0.01