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. 2020 Oct 27;18:169. doi: 10.1186/s12964-020-00660-4

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Stable knockdown of JARID1B repressed CRC proliferation via Wnt/β-catenin signaling pathway. a Gene set enrichment analysis of JARID1B based on TCGA COAD RNA Seq dataset showed that JARID1B expression was positively correlated with Wnt/β-catenin signaling pathway. b Western blot detected JARID1B, β-catenin c-MYC and cyclinD1 expression in DLD-1 cells transfected with shNC or shJARID1B. c The total and nuclear protein levels of β-catenin were assessed by western blotting in DLD-1 cells transfected with shJARID1B or shNC. d The inhibitive effect of JARID1B downregulation on Wnt/β-catenin pathway was detected by TOP-Flash luciferase reporter assay. e Western blot analysis showed the effects on c-MYC, cyclinD1 expression in DLD-1 cells when both JARID1B silencing and β-catenin restoration. f TOP-Flash luciferase reporter assay showing that β-catenin overexpression rescued the decreased Wnt/β-catenin pathway activity of DLD-1-shJARID1B cells. g, h, i EdU and in-vivo tumor formation assays showed that over-expression of ectopic β-catenin significantly rescued cells proliferation in DLD-1- shJARID1B cells. *p < 0.05